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KMID : 0613820110210050746
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Journal of Life Science
2011 Volume.21 No. 5 p.746 ~ p.752
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Antioxidative Activity and Chemical Characteristics of Leaf and Fruit Extracts from Thuja orientalis
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Ahn Hee-Young
Heo Su-Jin
Kang Min-Jung
Lee Jae-Hong
Cha Jae-Young
Cho Young-Su
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Abstract
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The contents of bioactive materials (e.g. polyphenolics compounds, flavonoids, minerals, and fatty acids) and antioxidative activities (DPPH (α,α"-diphenyl-β-picrylhydrazyl) free radical scavenging activity, peroxidation of linoleic acid and rat hepatocyte microsome, and Fe/Cu reducing power) were tested by in vitro experimental models using water, ethanol and methanol extracts of leaves (TOL) and fruits (TOF) from Thuja orientalis. Methanol extract from TOL showed the highest extraction yield (12.90%) as well as contents of polyphenolic compounds (16.02%) and flavonoids (0.25%). Major minerals were Ca, K, and Mg. Major fatty acids were palmitic and lauric acids in TOL and palmitic and decanoic acids in TOF. In oxidation of in vitro models using DPPH free radical scavenging activity, Fe/Cu reducing power, Fe²?/ascorbate-induced linolenic acid peroxidation by ferric thiocyanate and thiobarbituric acid (TBA) methods, and autooxidation of rat hepatic microsomes membrane, antioxidative activities were stronger in all extracts of TOL than in those of TOF in a dose-dependent manner. From these results, methanol extract of TOL was shown to have the most potent antioxidative properties and the highes content of antioxidative compounds such as polyphenolic compounds and flavonoids.
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KEYWORD
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Thuja orientalis, DPPH, antioxidative activity, polyphenolic compounds, flavonoids
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